were added to the sludge amended samples and compared to

those without primary substrates. The experimental scheme,

along with the multi-compound biodegradation assay, is

listed in Table 4-5.


Table 4-5. Experimental scheme for biodegradation and co-
 degradation studies of phenol, 2,4-DCP and PCP
 in multi-compound systems.


 No. Conc. N added Added Amendment NaN
 mg/l mg/l C:N (1 ml) mg/i

 401 5 0.74 10:1 2
 402 5 0.74 10:1
 403 5 0.74 10:1 sludge
 404 5 0.74 10:1 phegol
 405* 5 0.74 10:1 DCP
 406* 5 0.74 10:1 PCP
 407** 5 0.74 10:1 slg/acetate
 408 5 0.74 10:1 slg/glucosee
 409 1 0.15 10:1 2
 410 1 0.15 10:1
 411 1 0.15 10:1 sludge
 412 1 0.15 10:1 phegol
 413 1 0.15 10:1 DCP
 414 1 0.15 10:1 PCP
 415 1 0.15 10:1 slg/acetate
 416 1 0.15 10:1 slg/glucoseg

* Added with 0.5 ml of sludge supernatant at t=529 hours
** Discontinued at t=652 hours
a Solution that contains phenol degrading bacteria
b Solution that contains 2,4-DCP degrading bacteria
c Solution that contains PCP degrading bacteria
d 1 ml sludge plus 5 mg/l sodium acetate
e 1 ml sludge plus 5 mg/l glucose
f 1 ml sludge plus 1 mg/l sodium acetate
g 1 ml sludge plus 1 mg/l glucose


 PCP co-degradation in the presence of phenol. Previous

tests revealed that pentachlorophenol is very resistant to

biodegradation, and the presence of phenol seemed to

increase PCP's degradation. The PCP co-degradation in the