Hydraulic conductivity values were evaluated before and after a set of column biodegradation experiments. This test was designed to determine the effect of bacterial population increases on hydraulic conductivity. 4.2.3 Sludge Characterization Total volatile solids measurement. The following procedure provided the total volatile solids (TVS) measurements. Dry sludge weight and ash weight from 100 ml of wet sludge were measured after drying in an oven at 103 C overnight and combusting in a muffle furnace at 5500C, respectively (Sawyer and McCarty, 1967). TVS = ([wt. of dry solid] [wt. of ash]) x 10 (4-3) Organic carbon determination. In the sorption isotherm studies the organic carbon content of the sludge was determined in order to calculate K values. In sludges oc from municipal wastewater plants the volatile solids are mainly biomass. Accordingly, it was assumed that organic matter was the same as volatile solids, and corresponds to the biomass formula C60H 87023N12P (Benefield and Randall, 1980). Therefore, organic carbon, OC, is OC (mg/l) = TVS x (720/1374) = 0.52 x TVS (4-4) This factor, 0.52, agrees with the values 0.40-0.53 as suggested in Methods of Soil Analysis (Nelson and Sommers, 1982).