FLORIDA GEOLOGICAL SURVEY


utes as there are gallons of water remaining in tubes from the last sampling effort. FDEP
standard operating procedure for water quality sampling is then applied.

Water Samples

 Seven bottles and three whirlpacks were filled with water from spring vents and ana-
lyzed by the FDEP Bureau of Laboratories following Environmental Protection Agency or
Standard methods. All bottles were pre-rinsed with sample water prior to filling. Four bot-
tles and three whirlpacks were filled with unfiltered water samples. A GWV high capacity
in-line filter (0.45 /im) was attached to the microflex tubing and the remaining three bottles
were filled with filtered water samples. Bottles were filled in the order shown in Table 1.

 Table 1. Sampling order.

 Order Container Analyses Sample Preparation
 1 1 liter plastic BOD Unfiltered
 2 1 liter plastic Turbidity, Alkalinity, Color Unfiltered
 3 500 ml plastic Nutrient Unfiltered; H2SO4 acidification
 4 500 ml plastic Metals Unfiltered; HN03 acidification
 5 4oz whirlpacks (3) Bacteria Unfiltered
 6 500 ml plastic Anion, Alkalinity, Color Filtered
 7 500 ml plastic Nutrient Filtered; H2SO4 acidification
 8 250 ml plastic Metals Filtered; HN03 acidification

 Whirlpacks were placed on ice immediately after filling. Bottles for filtered and unfil-
tered nutrients (bottle nos. 3 and 7) were preserved with sulfuric acid followed by acidifica-
tion of bottles for filtered and unfiltered metals (bottle nos. 4 and 8) using nitric acid. pH
litmus paper was used to confirm acidity of pH less than or equal to 2. All water samples
were placed on ice and delivered to the FDEP Bureau of Laboratories within 24 hours.
Tubing and filters were discarded after each sampling event.

Additional Data

 General descriptions of each spring vent were made and included the aquatic, wetland,
and upland (where applicable) surroundings. Water depth was measured using a hand held
Speedtech sonar depth gauge. Distances were measured with a Bushnell Yardage Pro 500
range finder. Secchi depth was obtained using a secchi disk. A Trimble XR Pro GPS sys-
tem with a TDC1 data logger was used to record latitudinal and longitudinal coordinates.
Field parameter, weather conditions, sampling times, water and secchi depth, and
microland use information were also input into the GPS unit. Micro land uses within 300 ft
of spring vent were identified and sketched.

Discharge Measurement

 Where available, discharge data was obtained from the Water Management Districts,
U.S. Geological Survey (USGS), and published sources. Methodology for each discharge
technique is described below.