Step 4. In the analysis of variance for a 2" factorial, one degree of freedom is always assigned to each factor or interaction of factors because the factor effect is calculated by comparing two levels of the factor or interaction and one degree of freedom is lost in the estimation. Perform the analysis of variance (ANOVA): TABLE III-5. ANOVA for the 23 factorial Source of Degrees of Sum of Mean Fc variation freedom Squares Squares Blocks (b-l)=l 1.05 1.05 6.77 * Treatments (t-l)=7 28.46 4.066 26.23 ** Factor P 1 7.77 7.77 50.129 ** N 1 4.95 4.95 31.935 ** V 1 15.016 15.016 96.88 ** PN 1 .681 .681 4.39 NS PV 1 .0056 .0056 .036 NS NV 1 .000625 .000625 .004 NS PNV 1 .1056 .1056 .68 NS Error 7 1.084 .155 Total 15 30.594 CV = 6.38% * Significant at 5% level ** Significant at 1% level Interpretation of Results By examining the treatment mean yields in Table III-3, and the analysis of variance, Table III-5, it can be determined that each of the three factors individually (plant density, nitrogen, and variety) had a highly significant effect on yield. Out of the three factors, the new variety (V) produced the greatest increase (2.05 kg/plot), and plant density (P) was second with a 1.1 kg/plot increase. Although nitrogen (N) had a significant effect, increasing the yield by 21%, an economic analysis should be conducted to determine whether its application is an economically good choice. (Does the yield increase cover the cost of buying and applying the nitrogen