THE 2n-FACTORIAL TRIAL A useful arrangement of treatments that produces exploratory information on several factors and their interaction is a 2n-factorial trial. This is an arrangement of n factors taken at two levels each. An example of a 23-factorial experiment will be used to illustrate the detailed methodology of analysis. The nature of the trial was to explore three factors: plant density, nitrogen, and variety in a 23 factorial. The levels of the variables in this trial are given for plant density (P), nitrogen (N), and variety (V) as follows: PO = 25,000 plants/ha P1 = 50,000 plants/ha NO = None applied N1 = 100 kg N/ha V0 = Local variety V1 = Tuxpeio Table III-3 shows the field design by blocks and the grain yield in kg/plot. To estimate the factorial effects and perform the analysis of variance, the following procedure is applied: Step 1. Using treatment yield totals from Table III-3, a two-way table can be constructed in which the treatments are placed on the horizontal axis and the factorial effects are listed vertically as shown in Table III-4. In each of the factorial effects, half the treatment combinations receive a plus (+) sign and half receive a minus (-) sign. The row corresponding to M (mean of the entire experiment) has only plus signs. When the higher level of a factor (PI, N1, Vl) in the factorial effect is present in the treatment combination, it receives a plus (+) sign; it receives a minus (-) sign if not present (Po, No, Vo). When two or more factors at higher levels are present in the treatment combination, the sign is found by using the algebraic rule for signs.