or other means, and individual treatments are installed either by the researcher or the farmer. Together, the researcher and the farmer harvest the crop when it is mature. The design of a superimposed trial should be simple. Replications should be used at each location, although data from designs without replications at each site can be combined for regional analysis and interpretation. An example of a simple superimposed trial from IRRI is shown in Tables III-1 and III-2. Previous information indicated that rice responded to at least 50 kg/ha of nitrogen, but response to potassium and phosphorus was uncertain. A simple six-treatment superimposed trial was established on a number of farms and information was obtained from six of them (Table III-1). The design at each location was without replication. The six treatments included three levels of nitrogen (50, 70, and 90 kg/ha). At 70 kg/ha of N the treatments explored the application of 30 kg/ha phosphorus and potassium individually and together. Analysis of variance (Table III-2) indicated a significant effect for nitrogen but none for the other elements. The conclusion was that more nitrogen would have a positive effect on yield, and if the cost were less than the value of the additional crop, more nitrogen could be recommended. Furthermore, it would indicate that additional work should be done with nitrogen, but the other major elements (P and K) need not be studied further in this context. TABLE III-2. Analysis of variance of a superimposed N-P-K rice trial. Source d.f. SS MS Fc Total 35 69,071 Farms 5 32,178 Treatments 5 11,212 2,242 N 2 9,837 4,918 4.79 Remainder 3 1,375 458 0.45 Error 25 25,681 1,027 CV = 7.6% Source: Zandstra et al. (1981), p. 107