where Y comes from the previous equation. An follows: Treat- ments D E F III Ti ( ) 56 33 95 45 123 Bi 108 88 78 274 Y = [3(78) + 3(56) = 32 - 274] / (3 1) (3 1) The value 32 is used in block III for treatment D. For the analysis of variance, ANOVA (described in detail later in this chapter), total degrees of freedom (d.f.) is (rt 1) 1 = 7 and error degrees of freedom will be [(r 1)(t -1)] 1 = 3. Treatment sum of squares when calculated is reduced by [78 32(3 1)]2 / 3(3 1) = 32.67. Then treatment mean square and the F value can be calculated. Sum of squares calculated of missing plot value: with the estimate Source d.f. Sum of squares Blocks(r-l) 2 98.67 Treatments(t-l) 2 228.67 Error 4 10.67 Total rt-1 8 338.00 Note: r = Number of replications or blocks t = Number of treatments d.f.= degrees of freedom BLOCKS II 26 27 35 example