MICROBIOLOGY AND CELL .SCIENCE / 127 PHA 6471-Synthesis and Modification of Drugs (2) PHA 6905C Research Procedures in Medicinal Chemistry (1-4; max: 12) PHA 6934-Seminar in Medicinal Chemistry (1; max: 3) Weekly presentation and discussion of research reports based on college programs or literature. S/U option. MICROBIOLOGY AND CELL SCIENCE College of Agriculture GRADUATE FACULTY 1989-90 Chairman: E. M. Hoffman. Graduate Coordinator: S. R. Farrah. Graduate Research Professors: H. M. Johnson; R. R. Schmidt. Professors: P. M. Achey; H. C. Aldrich; J. E. Gander; G. E. Gifford; E. M. Hoffmann; D. H. Hubbell; L. 0. Ingram; R. A. Jensen;J. F. Preston, Ill; K. T. Shanmugam; P. H. Smith. Associate Professors: F. C. Davis, Jr.; D. E. Duggan; S. R. Farrah; W. B. Gurley; E. P. Previc; S. G.Zam. Assistant Professor: S. E. Coleman. Graduate study is offered leading to the Master of Science and Doctor of Philosophy degrees in microbiol- ogy and cell science, with emphasis in one or more of the disciplines of biochemistry, cell biology, and microbiol- ogy. Instruction and guidance are collaborative among faculty in the Colleges of Agriculture, Liberal Arts and Sciences, and Medicine. Areas of research include anaerobic metabolism; bio- mass conversion; nitrogen fixation; membrane and cell wall chemistry and biochemistry; immunology; virology; microbial ecology; bacterial and phage genetics; DNA, RNA, and protein synthesis; molecular aspects of devel- opment of procaryotic and eucaryotic systems; cell ultras- tructure. Prerequisites for admission to graduate study, in addi- tion to those of the Graduate School, are a broad educa- tional background including mathematics, physics, and chemistry through organic, analytical, and physical chem- istry; basic courses in botany and zoology; and preferably at least one course in microbiology. An undergraduate major in physical or chemical science, engineering, or general biology is usually acceptable. Receipt of an advanced degree requires detailed knowledge in micro- biology, biochemistry, and chemistry; undergraduate deficiencies may necessitate additional course work prior to entry into the graduate program. In addition to the courses listed below, the following will be accepted for credits toward graduate degrees in microbiology and cell science: BCH 6065, 6206, 6415, and BMS 6352C. MCB 5458-Energy Transformation in Microorganisms (3) Pre- req: CHM 3120; BCH 4313. Energy transformations of dissimila- tory and assimilatory processes in microorganisms with empha- sis on regulation and energy cycles. Applications of microbial energy transformations to low energy technology. MCB 6127-The Literature of Microbiology (2) Prereq: eight credits of microbiology. Bibliographic method in searching the literature. Literature of specific areas of the discipline. MCB 6315L-Recombinant DNA Techniques (2) Prereq: BCH 6415 and/or BMS 6352C and consent of instructor. Application of recombinant DNA techniques, agarose gel electrophoresis and hybridization for the in vitro construction, selection, and characterization of chimeric E. coli vector plasmids. MCB 6316-Plasmids, Transposons, and Recombinant DNA Technology (2) Prereq: BCH 6415 and/or BMS 6352C and consent of instructor. Background and currentstateof knowledge of plasmids, select bacteriophage, insertion sequences, transpos- ons, mobile genetic elements of eucaryotes and recombinant DNA methods. MCB 6415-Comparative Biochemistry and Physiology of An- aerobic Bacteria (2) Prereq: undergraduate biochemistry and microbiology and consent of instructor. Biochemical principles which govern energy flow through anaerobic food chains. Rumen discussed as a model system. Emphasis on selected microbial species and details of metabolism influencing, microbial interac- tion, nutrition, substrate dynamics, HB flow, methanogenesis. MCB 6416-Nitrogen Fixation and Inorganic Nitrogen Metabo- lism (2) Prereq: consent of instructor. Coreq: BCH 6206 and/or 6415. Biochemistry, physiology, genetics and regulation of nitro- gen fixation and enzymes of ammonia and nitrate assimilation. MCB 6435-Molecular Radiation Biology (2) Prereq: under- graduate courses in biology, chemistry, and physics, and consent of instructor. A survey of the effects of ionizing and nonionizing radiation, with emphasis on cellular and molecular mechanisms. MCB 6455-Temporal Gene Expression in Cellular Develop- ment (2) Prereq: undergraduate biochemistry and cell biology and consent of instructor. Sequential events in regulation of gene expression during cell differentiation; experimental systems and approaches. MCB 6456-Molecular Mechanisms in the Regulation of Tran- scription (2) Prereq: BCH 6415 or BMS 6352C and consent of instructor. Mechanisms regulating specific transcription in pro- caryotes and eucaryotes will be examined at the DNA and enzyme level. MCB 6485L-Advanced Techniques in Microbiology and Cell Science (2-4; max: 4) Prereq: consent of instructor. Application of advanced techniques to experimental research in biochemis- try, cell biology, and microbiology. MCB 6905-Experimental Microbiology (1'-8; max: 12) Prereq: eight credits in microbiology and cell science. Application of physical, chemical and biological techniques to experimental problems in microbiology. Individual laboratory study. H. MCB 6910-Supervised Research (1-5; max: 5) S/U. MCB 6930-Seminar (1; max: 8) Attendance required of all graduate majors at one research presentation each week as scheduled. S/U. MCO 6937-Special Topics in Microbiology (1-4; max: 12) Contemporary research in a particular aspect of general micro- biology. MCB 6940-Supervised Teaching (1-5; max: 5) S/U. MC8 6971-Research for Master's Thesis (1-15) S/U. MCB 7914-Research Conference (1; max: 8) Critical discussion and appraisal of research programs of faculty and students. MCB 7922-journal Colloquy (1; max: 8) Critical presentation and discussion of recent original articles in the microbiological literature. Attendance required. MCB 7979-Advanced Research (1-9) Research for doctoral students before admission to candidacy. Designed for students with a master's degree in the field of study or for students who have been accepted for a doctoral program. Not open to students who have been admitted to candidacy. S/U MCB 7980-Research for Doctoral Dissertation (1-15) S/U. PCB 5136L-Techniques in Microbial and Cell Biology (3) Pre- req: MCB 3020 and 3020L. A laboratory inexperimental bacte- riology and cell biology. Emphasis on experimental approaches and techniques used in study of cells and microorganisms. Experiments in microscopy, cell fractionation, metabolism, physiology, genetics, and regulation. PCB 5235-Immunology (3) Prereq: MCB 3020 and 3020L or introductory microbiology course which includes introductory immunology. Immune system of vertebrate animals. The cellular and molecular events involved inr immune responsiveness and resistance to infectious diseases. PCB 5235L-Experiments in Immunology (1) Prereq:MCB 3020L. Coreq: PCB 5235. Basic seriological procedures in immunology. PCB 6176-Electron Microscopy of Biological Materials (2) Prereq: MCB 3020 or equivalent; graduate student standing. Use of the electron microscope including fixation, embedding, sec- tioning, freeze-etching, negative staining, and use of vacuum evaporator. PCB 6176L-Laboratory in Electron Microscopy (2) Prereq: consent of instructor. Coreq: PCB 6176. Training in use of electron microscopes, ultramicrotomes, vacuum evaporators, and freeze-etch machines. PCB 6216C-Cytochemistry (3) Prereq: PCB 6176L. Cellular organization, cell function, and cytochemical technique. Given in even numbered years. BMS 6203-Molecular Biology and Function of Cell Membranes (2) Prereq: BCH 4203, 4313, and MCB 3020 or equivalent, and consent of instructor. Composition, molecular organization, and function of biological membranes in both eucaryotes and pro- caryotes. BOT 6256C-Plant Cytology (3) Fundamental structures of eucar- yotic and procaryotic cells, with emphasis on higher plants cells, algae, and cyanobacteria: