Everglades Station Mimeo Report EES64-18 1, ", 1964
 EVALUATION OF NEMATOCIDE TREATMENTS ON TURFGRASSN SOUTH FLOOR
 J. A. Winchester K


 Summary

 During 1963-64 21 commercial and experimental nematocide formulations made up
 more than 105 replicated treatments which were compared for control of plant nema-
 todes on 31 different turfgrass varieties. Only Zinophos and Bayer 25141 gave con-
 sistently good nematode control and increased plant vigor. Neither of these material
 caused injury to any grass tested. In one test an application of Bayer 25141 at 8
 and l6 pounds per acre (0.18 and 0.36 pounds per 1000 square feet) maintained
 Ormond Bermudagrass in a vigorous green condition for 12 months and controlled sting
 nematodes for 9 months. The granular and liquid formulations of zinophos and Bayer
 25141 appeared to be equally effective in all tests.


 Experiments
 Test No. Grass Location Nematodes
 1. Ormond bermudagrass PFL sting
 2. Everglades #1 bernudagrass PFL sting, ring, lance, spiral
 3. Meyer Zoysia Ft. Lauderdale sheath, sheathoid
 4. Bitter blue St. Augustine Delray cyst
 5. Zoysia matrella Lake Worth sting, lance
 6. Grass varieties test PFL sting, ring, lance, sheath
 7. Phytotoxicity summary So. Fla. burrowing and root knot



Application: Liquid nematocides were mixed in water and the granular nematocides
with sand to aid in application. All were applied uniformly to the plot areas.
Immediately after the treatments in each test were applied the entire plot area was
sprinkled with the equivalent of one quarter to one-half an acre inch of water in
order to move the nematocides into the root zone.


Sampling:
Nematodes
flotation


Plots were sampled periodically with a soil tube to a depth of 4 inches.
were removed from 100 ml. aliquots of soil from each plot by the centrifuge
technique.


Experimental design:
domized block design.
in which plots were 3


All treatments were replicated at least three times in a ran-
Plots in all tests were 3 feet square except in the first test
feet wide and 6 feet long.


Maintenance: All plot areas were treated periodically with fertilizers, insecticides
and fungicides to produce optimum growth.


 SThis report is a summary of the turf grass research conducted at the Plantation
 Field Laboratory, Everglades Experiment Station and other areas. It does not
 constitute a recommendation for the use of any nematocide without specific label
 clearance.

SAssistant nematologist, University of Florida, Everglades Experiment Station,
 Belle Glade, Fla.