Cultivar Identification reference values Certain biochemical analyses used in cultivar identification are not influenced by growing conditions and cultural prac- tices as are morphological measurements. Vermeulen et al. (26) found that it was possible to accurately identify FLoraTeXTM among 15 commercially available bermudagrass cultivars with use of starch gel electrophoresis. FLoraTeXTM produces a distinct "fingerprint" when stained for aconitase (Figure 1). Reproductive Characteristics FLoraTeXTM bermudagrass is a fertile, cross pollinated, tet- raploid perennial grass having a chromosome compliment of 36 (J.C. Read, TAES, Dallas, Texas, personal communication). Seedhead production disrupts normal vegetative growth and reduces turf quality. FLoraTeXTM produces seedheads, espe- cially during May and June. In an established turf in Gainesville, Fla., it produced 251 and 111 seedheads per square foot during May and June 1991, respectively. In May, 64, 33, and 3% of the seedheads had two, three, or four branches per inflorescence, respectively. In early June, 34, 51, and 1% of the seedheads had two, three, or four branches per inflores- cence, respectively. When counted in late June, 14% of the seedheads were immature, indicating a decline in seedhead production during that time. In a 1992 space-planted, polycross study at Gainesville, Fla., FLoraTeXTM had a seedhead production average of 85 seedheads per square foot and was equal to NuMex Sahara and Arizona Common which had an average of 109 and 73 seedheads per square foot, respectively Table 6. Average number and length of bermudagrass stolons per plug at 30 days after planting in a field on 21 May 1991 at Gainesville, FL. Ground cover rate and days to 50% ground cover of bermudagrass cultivars during the first 90 days are also presented (Dudeck, A.E., Unpub. Data). Stolon Ground cover Cultivar Number Length Ratet Cover, # cm % days FLoraTeXTM 46 ab* 4.7 a 41 a 484 Tifway 66 a 3.0 b 38 a 524 Tifgreen 21 bc 2.4 bc 28 b 623 Tifdwarf 19 c 1.1 c 18 c 966 t Biweekly nitrogen rate was 0.5 pound N per 1000 square feet. t Cover rate = sum of average weekly ground cover esti- mates for a 90-day period. Means within columns followed by the same letter are not significantly different (p=0.05) using Waller-Duncan k-ratio t test. doc A-22 A-29 Bayshore Everglades FLoraTeXTM Midiron Midway Ormond Pee Dee Santa Ana Sunturf Texturf 1F Texturf 10 Tifdwarf Tiffine Tifgreen Tifgreen II Tiflawn Tifway Tifway II Vamont o co LO r1 Figure 1. Isoenzyme banding patterns and reference val- ues for aconitase derived from starch electro- phoresis of crude protein extracts from turf-type Cynodon genotypes (After Vermeulen et al. 1991). (Table 7). FLoraTeXTM averaged 3.5 branches per seedhead, which was lower than branching in Arizona Common and NuMex Sahara, which averaged 3.8 per seedhead. After 13 years of observations in Texas, Sifers et al. characterized FLoraTeXTM along with Arizona Common, Everglades, Tufcote, and Vamont cultivars as "heavy" seedhead producers (24). Midiron, Midlawn, Ormond, Texturf 10, and Tifway were characterized as "light" seedhead producers. Sturkie in Ala- bama (12), Burton in Georgia (12), and Baltensperger in Ari- zona (2) all rated PI 213385 as being a heavy seedhead pro- ducer. In a NTEP test, FLoraTeXTM produced seedheads equal to that of Arizona Common, NuMex Sahara, Sonesta, and Vamont (14). This group had more seedheads compared to a group comprising Guymon, Midfield, Midiron, Midlawn, MS- Choice, MS-Express, MS-Pride, Texturf 10, Tifway, and Tufcote. There is a risk of turf contamination with off-types over time due to production of viable seed. Seedlings were obtained by Youngner in California from crosses involving P1 213385 both as a male or female parent indicating that FLoraTeXTM may have the potential to produce viable seed (12). In New Mexico, Baltensperger (personal communication) also pro- duced several open-pollinated progeny from crosses with PI 213385 and its off-types. In Gainesville, Fla., FLoraTeXTM had fewer seeds per seedhead compared to Arizona Common