BULLETIN FLORIDA STATE MUSEUM rest of the body, and present a definite golden caste in life (Raney, 1940a: pls. 1-4). The golden color disappears shortly after death, and the stripes again appear darker than the surrounding areas. Sim- ilar changes have been noted by Lachner (1952: 438) for Hybopsis biguttata. Other sexual differences are present at all seasons: The adult male has a deeper and more compressed head and body, longer pectoral and pelvic fins (table 6), a greater amount of fatty tissue on the fins, and reaches a larger maximum size (Raney, 1940a: 5). DISTIBUTION. Occurs over a large part of the northern half of the United States and southern Canada from northeastern Colorado, southeastern Wyoming, eastern parts of North and South Dakota and southeastern Saskatchewan eastward in the southern parts of the Hud- son Bay drainage, the upper Mississippi and Ohio valleys, and the Great Lakes-St. Lawrence drainage to the Atlantic coast as far south as south-central Virginia. On the Atlantic slope it ranges from the Gasp6 Peninsula and Nova Scotia as far south as the James River system in Virginia; it is common in the St. Lawrence and Great Lakes drainages and occurs in the southern part of the Hudson Bay drainage. The northern- most record is from a tributary of the Red River of the North in Man- itoba (Keleher, 1956: 265); the westernmost records are from Wyoming and Colorado. As indicated by Radforth (1944: 89) the northern limits of its range coincide closely with the 650 F. July isotherm. It occurs in the upper part of the Mississippi River drainage, including parts of the Missouri and Ohio systems, but is not recorded from the Missouri River system in Montana, and is known from this system in North and South Dakota only from the James River and other eastern tributaries. It occurs sparingly in the lower Missouri system of Nebraska, Iowa, and Missouri, with most records in central Mis- souri. In the Platte system it is known mostly from upland tributaries in northeastern Colorado, southeastern Wyoming, and western Ne- braska, with a few scattered records from the lowest parts of this drainage. In the Kansas River system it is abundant in places, the westernmost records being from isolated spring-fed streams. It is absent from the greater part of the Ohio River system, but is found in headwater tributaries in Indiana, Ohio, Pennsylvania and New York, being especially common and widespread in the Allegheny and Shenango drainages of New York and Pennsylvania. Isolated relict populations occur to the south in the lower Kanawha River system of West Virginia (Paint Creek, Fayette County), and in a tributary of the middle part of the White River in Indiana (Mill Creek, Hendricks and Morgan counties). Vol. 8