REPORT OF INVESTIGATIONS NO. 42 bicarbonate. Additional ground-water inflow in this reach of the river is indicated. The chemical characteristics of this inflow indicate that it was derived from the Floridan aquifer. Gator Hole Slough, a tributary to the Withlacoochee River downstream from Dade City, was sampled at high flow. The water was low in mineral content and contained sodium and chloride as the principal dissolved mineral constituents. The color was 180 units. Data were collected during the period of low flow (May 23-25, 1961) to determine the quantity and mineral content of the ground-water inflow in the reach of the Withlacoochee River between the stations near Lacoochee and Trilby (see fig. 11). The mineral content of the ground-water inflow from the Floridan aquifer into this reach of the Withlacoochee River was computed using the load equation (Hem, 1959): QIC, + Q2C2 Q3C3 where, Q is the discharge in cfs C is the mineral content in ppm QiCi is the instantaneous load near Lacoochee Q2C2 is the instantaneous load between data- Collection stations QsC3 is the instantaneous load at Trilby The inflow (Q2) was determined to be 12.2 cfs by subtracting the discharge near Lacoochee from that at Trilby. The mineral content (C2) of the inflow was then computed to be 260 ppm which is approximately equal to that of water in the Floridan aquifer in this area. The mineral content of water in the Withlacoochee River at Croom (station 44) was less than that at Trilby (station 42) or that at Rerdell (station 43). The difference between the sum of the discharges at Trilby and Rerdell and that at Croom on May 25, 1961, was 38.9 cfs (see fig. 11). Based on the load equation, the mineral content of the inflows between the stations would be 148 ppm. Similar computations of data during other periods show the mineral content of the ground-water inflows in this area to range from 148 to 174 ppm. The computed mineral content indicates that the inflow between stations was probably a composite of surface water and ground-water inflows. The mineral content of the water in the Little Withlacoochee River is shown in figure 13f. The color was usually above 100 units during periods of high flow. The principal mineral constituents during periods of low flow were calcium-and bicarbonate, the water