Use of Open Versus Closed Systems in Caribbean Prawn Hatcheries Dallas E. Alston Department of Marine Sciences University of Puerto Rico-Mayaguez Campus Mayaguez, PR 00708 USA The culture ofMacrobrachium rosenbergii, the giant Malay- sian prawn, has led to Caribbean culturists starting hatcheries to produce postlarvae as stock. Two systems are primarily employed: open, which utilize continuous or batch exchange of water, and closed, which recirculate the water. Open systems are generally safer since water exchanges can be made as needed. A disadvantage of open systems is that the hatchery El cultivo de Macrobrachium rosenbergii, el camar6n gigante de Malasia, ha obligado a los cultivadores del Caribe a empezar sus propios criaderos para la producci6n de postlarvas para la cria. Los sistemas abiertos y cerrados son los dos prin- cipales metodos usados. Los sistemas abiertos utilizan flujo continue del agua o cambios frecuentes de esta. La ventaja de este sistema esta en el poder eliminar aguas contaminadas Mrcrobrachium rosenbergii, the giant Malaysian prawn, has become an increasingly important culture organism in the Carib- bean region. Currently, there are prawn farms in Costa Rica, the Dominican Republic, Honduras, Jamaica, Martinique, Panama, and Puerto Rico. Most of the culturists prefer to establish a local hatchery to produce their own stock of postlarvae since importa- tion on a large scale is prohibitive. Macrobrachium hatcheries require brackish water with salinities ranging from 12 to 19 ppt. Two systems are primarily employed: open, which utilize continuous or batch exchange of water, and closed, which recirculate the water. There are many designs and methods for culture. The choice of a suitable system should be determined by the availability of a good water source, the distance from the hatchery to the growout ponds, and the in- itial investment. Open Systems Open systems are often a logical choice for the majority of prawn hatcheries since the initial investment is often lower than for closed systems. A good supply of uncontaminated fresh and saline water is needed. The site of the open system thus necessitates that the hatchery be located near a saline water source, since considerable amounts of water are used in con- tinuous or batch exchanges of water. The intensive labor involved in the culture of the larvae results in considerable capital input during the culture period. However, if problems arise during the culture, an open system provides for complete exchange of the culture water, a practice which may often save the larvae. The hatchery should have a total water storage of at least dou- ble the larval tank volume. This capacity allows for adequate water storage for the mixing of the brackish water and provides space for storing postlarvae before distribution to ponds (New and Singholka, 1982). VOL. XX-PROCEEDINGS of the CARIBBEAN FOOD CROPS SOCIETY Fernando Ibanfez Aquaculture Enterprises, Inc. Call Box 5000 Caja 449 San German, PR 00753 USA must be located sufficiently near a source of saline water. Closed system hatcheries can be situated far from the coast, since the addition of both fresh and saline water is minimal. Disadvantages of closed systems are the higher initial costs and difficulty in eliminating contaminated waters. en un moment determinado. Los sistemas cerrados recirculan el agua. Los criaderos de sistema cerrado pueden ser instalados lejos de la costa, ya que el uso de agua dulce y salada es minimo. Las desventajas de este sistema se deben al incremen- to del costo inicial y al problema de deshacerse del agua de cultivo cuando surgeon problems. Los sistemas abiertos utilizan cambios continuous de agua o en poca cantidad. Surface water should be filtered before use in the hatchery. The filtration process should include a sand filter and then a smaller mesh filter near the mixing tank (New and Singholka, 1982). Tap water can often be used without filtration, since most of the water has been treated for human use. Care must be taken, however, to remove the residual chlorine from the water. Ideal sites would be where the wells could be drilled to various depths to either saltwater or freshwater strata (New and Singholka, 1982). This could be done along the coast where salt- water intrusion occurs and forms a wedge beneath the freshwater strata. Care must be taken if surface water supplies for fresh or saltwater are to be used. These waters are subject to contamina- tion and salinity variations. If the hatchery location requires use of surface water, the water should be carefully checked for pesticides (New and Singholka, 1982). Other water quality parameters should also be monitored. Prawn larvae require water of extremely high quality because they are particularly susceptible to toxic ammonia, and to bacterial and fungal infections (Aquatic Farms, 1979). Postlarvae are especially susceptible to nitrite and nitrate, both in terms of acute and chronic toxicity (New and Singholka, 1982). Sublethal effects of nitrite at levels as low as 1.8 ppm with prawn larvae have been reported. Reports are that hardness of less than 100 ppm CaCO3 are suitable for freshwater (New and Singholka, 1982). Two techniques of open systems are generally employed in prawn hatcheries. These two methods have been described by Aquatic Farms (1979) as follows: Green Water Method Phytoplankton is cultured in separate tanks to precondition the water prior to introduction into the larval rearing tanks. Every other day, the phytoplankton-rich water is used to replace 50 to 75% of the volume in the larval tanks. With this method, the phytoplankton continuously "condition" the water (presumably by taking up the metabolic wastes of the larvae and other food organisms), and shade the larvae from strong sunlight. 45