112
Mutant RNA Substrate Wild Type RNA Substrate Time(hours) 0 1.5 3.5 6 13 24 52 100 0 1.5 3.5 6 13 24 52 100 Substrate .
Product
Figure 50. The HH 1 hammerhead ribozyme cleaves a 32P-labled RNA oligonucleotide with the mutant but not the wild-type sequence. This figure is a scan of an autoradiogram of a 15% acrylamide 8 M urea gel. The substrate is 13 nucleotides and the product is 7 nucleotides.