30
on a molar basis, for the process is
K = [BHP]/[BH][Su]q
(1-16)
where [BHP] is the molar concentration of bound species,
[BH] is the concentration of free small molecule, and
[Su] is the concentration of the uncomplexed DNA sites.
Unbound site concentration may be determined from the
total site concentration, S^, and bound site concentration,
S
c
(1-17)
Now, since
Sc/q = [BHP] (1-18)
and st = [Pt/m] (1-19)
where Pfc is total DNA phosphate concentration, we may
write
su = [pt/m] qtBHp]
(1-20)
Substituting equation (1-20) into (1-16) yields
K =
[BHP]
[BH]([Pt/m]- q[BHP])q
(1-21)
Taking the log of both sides of (1-21) and rearranging,
we obtain
109 ( !bh]] = log K + qlog ([pt/ml- q[BHP])q (1-22)