CONCLUSIONS It is apparent that further research will be necessary to elucidate the mechanism causing the fatigue observed in the experiments reported herein. This research, however, has eliminated several proposed mechanisms of fatigue. A few hypotheses are presented which may contribute to the fatigue observed in these experiments. Each of these hypotheses is based on a central theme, a reduced activation of the contractile proteins. It remains to be seen which of these mechanisms (if any) is the actual cause of the twitch fatigue observed in these experiments. 95