Fig. 3-2. Linearization of the curved Scatchard plot shown in Fig. 3-1.
The same hypothetical data points presented in Fig. 3-1 are shown plotted in the
coordinate system recommended for the determination of K^. The ordinate (B^/F^) now
refers explicitly to the concentrations of bound and free competitor (E^), and the
concentration of free binding sites (BQ-BL-BC) is plotted on the abscissa. The plot must
pass through the origin, and the slope (l/KdC) is the reciprocal of the dissociation
constant of the competitor (E^). The inset is analogous exactly to the inset of Fig. 3-1
the points are plotted with the total concentration of bound sites (B^B^) on the x-axis.
In this "Scatchard format" the slope is (-1/K^) and the x-intercept is again Bq.