147
Table 27
Effects of Pronase Digestion on Membrane Associated
Immunoglobulins of Bluegill and Mouse Lymphocytes
CPM x ID'3 Precipitateda
Experiment
Cells
Treatment
NRS
Ra-Ig
1
Bluegill Kidney
None
14.0
41.0
Mock Pronase
13.3
38.9
Pronase
14.2
40.5
Bluegill Kidney
None
8.5
39.5
Mock Pronase
7.4
37.0
Pronase
8.5
38.0
5
Mouse Spleen
Mock Pronase
2.0
11.2
Pronase
2.1
2.0
(a)Treated cells were lyzed with Nonidet P-40 and the lysates were pre
cipitated with either normal rabbit serum (NRS) or rabbit anti-bluegill
immunoglobulin (Ra-Ig).