116
Table 24
Cytoplasmic Immunofluorescence Studies of Uncultured
and Cultured Alligator Peripheral Blood Lymphocytes
% Positive*3
Cells3
NRS
Anti-Ig
Uncultured Control
0
0.1-0.3
Cultured Control
0
0.5-1.5
Cultured with PHA (1 yl)
0
0.5-1.0
Cultured with LPS (10 yg)
0
7-10
(a) Cultured cells were incubated with or without mitogens at 32C for
five days. Cytocentrifuged slides were prepared of cells obtained from
cultures or Hypaque-Ficoll isolates of uncultured cells and were stained
for cytoplasmic immunoglobulin.
(b) The number of positive staining cells is expressed as a percent of
the total number of white cells counted. The "sandwich" stain was nor
mal rabbit serum (NRS) or rabbit anti-alligator immunoglobulin (Anti-Ig)
and the second stain was fluorescein conjugated goat anti-rabbit IgG.