Table 23
Depletion of LPS and PWM Responsiveness
in Alligator Peripheral Blood Lymphocytes Passed
Through an Anti-Immunoglobulin Immunoadsorbent
Stimulation
Index
cl
Treatment
% Recovered'3
LPS
PHA
PWM
Unfractionated
-
5
74
9.8
NRS Immunoadsorbent
100
5.5
80
12.5
Anti-Ig Immunoadsorbent
67
1
71
1
(a) Immunoadsorbents were prepared from 40% saturated ammonium sulfate
precipitates of rabbit anti-alligator immunoglobulin (Anti-Ig) or normal
rabbit serum (NRS) coupled to Sephadex G-200.
(b) The number of cells recovered is expressed as a percent of the
total number of cells applied to the immunoadsorbent column.
(c) Cultures were incubated at 32C, pulsed on day 4 and harvested on
day 5. Mitogen concentrations were 10 yg, 1 yl and 10 yl for LPS, PHA,
and PWM respectively.