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protein derivative (a gift from Dr. R. Waldman, University of Florida)
were used,and 3) only peripheral blood lymphocytes were used in in vitro
primary immunization studies.
Assay for %-Thymidine Incorporation into DNA
Assay techniques are described in Chapter II.
Stimulation Indices and Statistical Analysis
Statistical analysis and formulas for calculating stimulation
indices are presented in Chapter II.
Autoradiography
Techniques for autoradiography are presented in Chapter II.
Histological and Morphological Techniques
Serial cross sections of paraffin embedded organs were kindly pre
pared by Mr. Larry J. McCumber (Whitney Marine Laboratory, Marineland,
Fla.). Sectioned tissues, as well as cytocentrifuge preparations of
cell suspensions, were stained with May-Grunwald-Giemsa stain.
Preparation of Rabbit Antisera
The brain of one sacrificed alligator was used for immunization
purposes, following techniques described in Chapter II. Antisera from
two rabbits immunized and boosted eight times over a four month period
were used. Preimmune sera from the same rabbits were used as normal
rabbit serum controls.
Rabbit anti-alligator immunoglobulin was prepared by immunizing
rabbits with immunoglobulins isolated by Sephadex G-200 (Pharmacia)
column chromatography. An ammonium sulfate precipitate of alligator
serurn was applied to the column.