43
Table 7
Mitogen Responses of Bluegill Anterior Kidney
Lymphocytes Treated with Anti-Brain Plus Complement
or Rosette Depleted with Rabbit Red Blood Cells
Stimulation Index3
Expt
1
Expt
2
Expt
3
Treatment
LPSb
PHA
LPS
PHA
LPS
PHA
Control
4.2
9.8
4
12.4
8
9.9
Anti-Brain + Complement0
30
1.3
26
1.3
ND
ND
Rosette Depletion^
1.8
17
NDe
ND
1
9.0
(a) Triplicate cultures incubated at 32C, pulsed on. day 6 and harvested
on day 7.
(b) Mitogen concentrations were 10 yg and 0.1 yl of LPS and PHA respec
tively.
(c) A 1:5 of rabbit anti-bluegill brain plus a 1:10 of guinea pig com
plement was used in the cytotoxic treatments.
(d) Rabbit red blood cell rosetted cells were depleted by Hypaque-Ficoll
centrifugation.
(e) ND = Not Done.