41
Table 6
Mixed Lymphocyte Responses of Bluegill
Anterior Kidney Lymphocytes
Stimulation Index3
Mixed
Lymphocyte
Response
Mitogen
Response
22C
32C
Fish
Crossc
22C 32C
LPSb
PHA
LPS
PHA
1
1 + 2
1 25
62
2.3
9
95
2
17.2
7.2
8.9
145
3
3+4
1 12
22
16
3.4
51
4
20
3
2.4
55
3
3+5
1 9
22
16
3.4
51
5
24
2.9
5.3
22
4
4+5
1 19
20
3
2.4
55
5
24
2.9
5.3
22
(a)
Triplicate
cultures were pulsed
on day
6 and
harvested
on day
Results are expressed as stimulation indicies as defined in Materials
and Methods.
(b) Mitogen concentrations were 1 yg and 10 yg of LPS at 22C and
32C, respectively, and 0.1 yl of PHA at 22C and 32C.
(c) Designates the source of cells used in the mixed lymphocyte
cultures.