30
Table 4
Effect of Maintenance Time of Bluegill in Laboratory
Aquaria on the Incorporation of Thymidine by
Unstimulated Anterior Kidney Cell Cultures
CPM/Culturea
Fish^
Maintenance Timec
22C
32C
A
1 day
508
4518
B
1 day
120114
4416
C
3 weeks
1121137
26451109
D
3 weeks
991168
12311127
E
5 weeks
13,1971580
28381181
(a) Results are expressed as the means of CPM from triplicate
cultures  standard deviations.
(b) Cells from individual fish were incubated without mitogens
at 22C or 32C, pulsed with ^H-thymidine on day 2 and harvested
on day 3.
(c) Length of time fish were maintained in laboratory aquaria
before sacrifice.