26
Table 2
Effect of Dialysis of Plasma Supplements on Mitogenic
Stimulation of Bluegill Anterior Kidney Lymphocytes
Stimulation Indexa
Supplement Plasma
Poolb
PHA (0.1 yl)
- 32C
LPS (1 yg)
- 22C
Undialyzed
Dialyzedc
Undialyzed
Dialyzed
A
1
6.8
1
1
B
4.8
4.0
1
14.0
C
2.5
4.0
5.9
16.4
D
4.8
4.6
1.5
7.4
(a) Triplicate cultures were stimulated with either PHA (0.1 yl) at
32C or LPS (10 yg) at 22C, pulsed on day 6 and harvested on day 7.
(b) Each pool represented the plasma obtained from at least five bream.
(c) Dialysis vas against pyrogen free 0.15 M NaCl.