94
In this way all elution volumes are referenced against the volume at
which E elutes when [I[ =0.
Taking a double reciprocal yields,
1 1
II] + Kj
Ve V VE=H
'ES .7
[I]
(20)
Separating terms on the right side gives an equation of the form
y mx + b,
1
K!
1
Ve % "
'KE1 '
+
[x]
kt=m
VL-M
k=h
J
*£=* "
1
(21)
Thus a plot of
VP V
versus
i
[i]
would give a straight line with
and,
Slope =
kt=h
VL-M
*9*
(22)
ordinate intercept
iTC=n
Kr=?r
i
j
(23)
Further,
Slope
ordinate intercept
K.,
(24)
L-M
Finally, substitution of V VD for the value of V-
(21) will give the ratio of It to K~ .
L-M L-M
into equation