52
When there is no interaction with the matrix by the enzyme equation (7)
becomes,
D =
0 Vg
(10)
where VQ is the elution volume for this case. Substitution of (10) into
(9) gives,
.MI
Ky-ZF
ve vm = (V0 Vm) + (Vo Vm) Syr + (Vo Vm)
1 + Kt
rearrangement and simplification gives,
nurm 0
(14)