102
Table 3-2
3H-thymidine incorporation:
Monolayers^
Cell Type
Marker
Labelled Nuclei
Flat
GFA/5A11
+
Neuron
A2B5/NF
-
Oligodendrocyte
GC
-
Abnormal
A2B5/GFA/
5A11/NF
-
a 3H-thymidine incorporation into monolayer cultures
exposed to continuous labelling. Of the cell phenotypes
described below, the only one that contained labelled
nuclei was the A2B5(-) flat cell. This demonstrates that
new DNA synthesis is not required for either recruitment
of A2B5(+) cells from A2B5(-) cells or their
differentiation. Similarly, cells that developed into
neurons or oligodendrocytes did not need new DNA
synthesis in culture.