Fig.3-7. 3H-thymidine autoradiography combined with
immunohistochemistry of monolayer cultures after 8 days in
vitro. Cultures were continuously incubated in culture
medium containing label, and dipped slides were exposed for
5 days before developing. Micrograph sets are comprised of
(from left to right) phase contrast, brightfield, A2B5
immunostaining, and (a only) GC immunostaining. All black
is autoradiographic label in the unstained brightfield
micrographs, and nuclei appeared as large densely labelled
areas. In both unpurified and purified cultures labelled
nuclei were present only in A2B5(-) flat glia. A2B5(+)
neurons (a), A2B5(+) nonneuronal cells (b), and GC(+)
oligodendrocytes (a) did not contain labelled nuclei. Bar,
50nm.