Fig.3-6. In vitro development of oligodendrocytes as
defined by immunostaining with antibodies against GC. Shown
are micrograph sets of cultures double-labelled for GC and
A2B5. GC(+) oligodendrocytes developed in cultures from
both unpurified (a) and purified (b) cells and were always
A2B5(-). Oligodendrocytes frequently extended processes
that were in close contact with A2B5(+) neurons (a). A
large aggregate of neurons is demarcated by the "a". In
purified cultures GC(+) oligodendrocytes were more numerous
than in unpurified cultures. Shown in (b) is an
oligodendrocyte in the same field as several A2B5(+) cells.
Oligodendrocytes tended not to be in close proximity to
A2B5( + ) nonneuronal cells. Bar, 50jim.