Fig.3-5. Expression of neurofilament reactivity (b,d,e) and
glial filament reactivity (a,c) in 1-2 week old monolayer
cultures of unpurified (a,b) and purified (c-e) cells. GFAP
reactivity in unpurified cultures (a) was confined to the
majority of flat A2B5(-) glia that underlie the networks of
single and aggregated (labelled "a") neurons. In purified
cultures (c) GFAP reactivity was also found in the A2B5(+)
nonneuronal cells (arrowheads). An A2B5(+) cell with
extended processes (white arrow) also appears to react with
GFAP antibodies. Neurofilament reactivity in unpurified
cultures (b) were present in neurons (mainly A2B5(+)) and
not in the A2B5(-) glia. In purified cultures (d,e) NF
reactivity also was found in some flat cells that were
A2B5(-) or low level A2B5(+). Distinct filaments could be
seen in these cells as shown in a higher magnification of
several cells from a different field in (e). Bar (a-d),
50p.m. Bar (e), 25pm.