Fig.3-4. In vitro development of A2B5(+) cells in monolayer
cultures made from unpurified (a-c) and purified (d-f)
cells. Shown are phase/ fluorescent pairs with the
antibody staining shown on the right. A2B5(+) cells in
unpurified cultures appeared solely as neurons after about
a week (a) or 2 weeks (b) in vitro. In these cultures, the
antibody 5A11 reacted only with the flat A2B5(-) glia (c).
In purified cultures, the A2B5(+) cells appeared
predominantly with a flat glial morphology after about a
week in culture (d) and were rounded up after several more
days or when the monolayers approached confluency (e). Some
A2B5(+) neurons with processes also developed in these
cultures. The A2B5(+) nonneuronal cells reacted with 5A11
(f, arrowheads) revealing a partial glial phenotype. The
micrograph pair in (g) shows several A2B5(+) nonneuronal
cells in a monolayer culture that was made from unpurified
cells that were suspended in methyl cellulose medium for 24
hours prior to plating. Thus these cells can be generated
in culture by 2 different isolation procedures. Bar, 504m.