Fig.3-3. Analysis of initial purification of A2B5(-) cells
and A2B5 antigen modulation on purified cell surfaces by
immunofluorescence (a) and immunoradiometric (b) assays.
The immunofluorescent assay (a) indicated that the purified
population of cells was initially devoid of A2B5(+) cells.
After 24 hours in culture, however, the purified population
was 80% A2B5(+). The unpurified population did not show
any increase in A2B5(+) cells. The immunoradiometric assays
(b) confirmed that the purified cells were devoid of cell
surface A2B5 antigen initially (left graph). After one day
in culture the purified cells expressed levels of A2B5
antigen approaching those of unpurified cells (right
graph). Shown in all graphs are means  s.e.m. DPMs are
higher in the +1 day immunoradiometric assay due to use of
125I-labelled antibody with 3x the specific activity.