Fig.3-1. Graph comparing the cell surface reactivity of
marker A2B5 to reactivity against NF, GS, and GC. The
percent of total dissociated cells that react with each
antibody is quantitated on the abscissa. The percent that
react with purified cells is obtained by multiplying the
percents on the A2B5(-) side by 2. A2B5 bound to
approximately 50% of dissociated cells as shown on the top.
Anti-NF reactivity was present in =12% of the cells and 10
out of 12 of these were A2B5(+) (this data only is from
days 7 and 8 cells; see text). Anti-GS antibodies reacted
with =40% of total cells and all of these were A2B5(-).
Similarly, anti-GC antibodies reacted with only A2B5(-)
cells (6%) and therefore were enriched in the purified
fraction. Approximately 95% of the purified cells could be
identified as reactive with the markers NF, GS, and GC.
More than 90% of the purified cells could be identified as
either GS(+) astrocytes or GC(+) oligodendrocytes.