39
Table 2-1
Effects of substrata on monolayers
Cells
Substratum
Growth
Unpurified
Glass
All cells
Plastic
r r
Aclar
r r
Collagen
r f
Polyornithine
Neurons only
Purified
Glass
All cells
Plastic
/ /
Aclar
/ f
Collagen
t t
Polyornithine
No growth
Microsphere
Glass
All cells
Plastic
r r
Aclar
/ /
Collagen
/ r
Polyornithine
Neurons only
Mixed
Glass
All cells
Plastic
/ r
Aclar
r r
Collagen
/ r
Polyornithine
Neurons only
 Effects of substrata on the growth of neurons and glia
in unpurified and purified monolayer cultures. The only
substratum out of those tested which was cell type
selective was polyornithine which was selective for
neurons. Practically all cells degenerated on
polyornithine in cultures made from purified A2B5(-)
cells.