Fig.2-6. Experimental design and results of controlled
incomplete separations. Plain dissociated cells were mixed
in some ratio with those that were incubated in A2B5 and
then the vital fluorescent dye DiO (diagram). After the
immunomagnetic separation cells were plated on
polyornithine. After 1 day in culture cells were
immunostained with A2B5 and scored were the % of DiO(+)
cells that had become A2B5(+), except with the leftmost
points where none of the cells were incubated in dye or
A2B5. Those cells were scored for %A2B5(+) cells initially
and after one day in culture. Plating efficiencies after
one day in culture approached 100%. The results graph shows
that as a greater percentage of the A2B5(+) cells are
removed from dissociated cells, a greater percentage of the
known A2B5(-) cells convert to A2B5(+) cells. If no A2B5(+)
cells are removed then only a slight increase in A2B5(+)
cells occurs. The (2) indicates two identical results at
that point. The results indicate that A2B5(+) cells are
recruited from A2B5(-) cells as a function of the depletion
of A2B5(+) cells.