Fig.2-4. In vitro development of A2B5(+) cells in both
unpurified (a-c) and purified (d-f) cultures. Initially
after the separation the unpurified cells (a) were =45%
A2B5(+) while the purified cells (d) were devoid of A2B5(+)
cells. After one day in culture both the unpurified (b) and
purified (e) cells were =50% A2B5(+). Similarly, monolayer
cultures after approximately 1-2 weeks contained A2B5(+)
cells with neuronal morphologies. A2B5 did not react with
the flat cells underlying the neurons. Shown are monolayers
after 8 days in culture at high magnification. The fields
of neurons shown in c) and f) were chosen to illustrate the
morphologies of the A2B5(+) neurons and not to show the
equal densities of neuronal clusters that were present.
Bar, 50(im.