Fig.2-1. Separation chamber used for the immunomagnetic
separations and Nomarsky micrographs showing cells before
and after the purification. Construction was of plastic
tubing and funnel held on a ring stand. Two magnet arrays
were used to ensure collection of all of the paramagnetic
microspheres. A pinch clamp was used to control the rate of
flow through the chamber. After ethanol sterilization in a
sterile hood the cell-microsphere mix was poured into the
funnel and the purified cells were collected in centrifuge
tubes from the bottom. Micrograph (a) shows smaller
microspheres binding to one cell (arrowhead) but not to
another. Micrograph (b) shows cells that were removed from
the mixture due to their coating of microspheres
(arrowheads). Bottom micrograph (c) shows 6 purified cells
without attached microspheres. Bar, 50nm.