Figure 12. ION EXCHANGE CHROMATOGRAPHY ON DEAE-SEPHADEX A-25
3
The procedure is described in the methods. The RNA polymerase activity profile when H-UTP was
substrate is represented by the solid line while the profile when ^h-CTP was the substrate is
represented by the broken line ( A A ). The A280 Pro;file is represented by the double
line while the ammonium sulfate concentration in each fraction is represented by the heavy dashed
line.