Figure 20. Map of Ddel restriction sites identified in honey bee genomic DNA
insert of probe pB178. The Pstl sites marking the ends of the insert were
utilized for cloning in pBR322. Relative positions of sites were determined by
digesting smaller regions of the insert (Pstl, Kpnl, Kpnl Hindill, Hindill Bgill,
Bg/ll EcoRI4, and EcoRI4 Pstl2) with Ddel in the absence and presence of
restriction enzymes within each region, which were indicated in Figure 12 (with
the exception of the region between the Kpnl and HindIll sites). The asterisks
(*) indicate sites identified by sequencing the ends of the respective subclones.