730


 1110
 1220


 1800




 2220


 2835
 279 -
 2880




3630










6000 -


?8 =


5770 -
8020 -











7680



8140


 Pstli





 Mspl





 Mspl



- Mspl


- KpnI





 Hindlll

WfI


 Mspi

 ~MspI *
 BgfiI

 MspI
 Mspi









 EcoRI





- MspI








 MsPI
 Pspti


1250bo


1400bp


1300bp