Figure 13. Map of Mspl restriction sites identified in honey bee genomic DNA
insert of probe pB178. The Pstl sites marking the ends of the insert were
utilized for cloning in pBR322. Relative positions of sites were determined by
digesting smaller regions of the insert (Pstl, Kpnl, Kpnl Hindlll, HindIll Bg/ll,
Bg/I EcoRl4, and EcoRI4 -Pstl2) with Mspl in the absence and presence of
restriction enzymes within each region, which were indicated in Figure 12
(with the exception of the region from the Kpnl site to the Hindlll site). The
asterisks (*) indicate sites identified in the DNA sequences of the ends of the
respective subclones. Left: distances (in base pairs, bp) between Mspl sites.