51
 these east and west European populations. There was little variation in the

 restriction fragment patterns within each European sample population.

 Examples of Mspl fragment patterns detected in A. m. ligustica workers

 are shown in Figure 9, lanes 1-3. The fragments detected corresponded to

 M100 and M200 variants, with estimated group frequencies of 81% and 19%,

 respectively (Table 4). The majority of A. m. ligustica workers appeared to

 have two M100 variants (Figure 9, lane 1), while the A. m. caucasica workers

 examined all appeared to have two M200 variants (Figure 9, lane 3). The Ddel

 fragment patterns for the A. m. ligustica and A. m. caucasica workers

 examined (not shown) contained 1.25kb and 0.75kb fragments characteristic

 of variants in the D300 group (Figure 11). It is possible that D200 variants

 were present but could not be detected due to comigration of D300 fragments

 (see Figures 4 & 11).

 Examples of Mspl fragment patterns detected in A. m. mellifera workers

are shown in Figure 9, lanes 4-7. All the A. m. mellifera workers examined

appeared to have at least one M300 variant. The frequency for the M300

variant group was 94% (Table 4). The majority of the workers appeared to

have two M300 variants (Figure 9, lanes 6 & 7). The remaining A. m. mellifera

workers appeared to have an M300 variant together with an M100 or M200

variant (Figure 9, lanes 4 & 5, respectively). All Ddel fragment patterns

detected in the A. m. mellifera workers contained a 1.25kb fragment

characteristic of the D200 and D300 groups (not shown). The majority