ALLELE FREQUENCIES AT LOCUS 178 REVEAL HYBRIDIZATION
 OF EUROPEAN AND AFRICAN BEES IN THE NEOTROPICS


 Introduction


 Frequencies are reported here for the individual variants, for the groups

 of variants with common fragments and distributions, and for the alleles found

 at locus 178 in Old and New World populations of honey bees. Unlike previous

 nuclear DNA markers (Hall 1990, 1992b), variants and alleles detected at locus

 178 appeared to be specific to east European, west European, or African bees.

 Ancestry in New World bees was inferred from the frequencies for the variants,

 variant groups, and alleles. The results reveal a level and specificity of African-

 European hybridization not observable in previous DNA studies.



 Materials and Methods


 Procedures for the isolation and electrophoretic analysis of honey bee

genomic DNA, identification of the source and size of probe pB178 used for the

detection of RFLPs, and the details of the initial detection of polymorphisms,

were given in the proceeding chapter. RFLPs were initially detected in pooled

sibling samples of worker bees. For determining the frequencies for the Mspl