31 Honduras, Ddel variant D303, found in three alleles, was associated with Mspl variants M102, M103, and M201, indicating European origin. The presence of Ddel variant D404 in the USA, Honduras, and South Africa was an exception to the correlation of fragment pattern with geographic origin. The alleles with D404 were differentiated by Mspl variants, which were consistent with the sample origin and what was known of its ancestry: M302 (USA), M514 (Honduras and South Africa), and M515 (South Africa) (Table 2). Variants in the M300 group were found in all populations examined, although individually they appeared to be limited in distribution. Variant M302, which contained a 0.75kb fragment not present in M301, M303, and M304, was found only in the USA and Mexico, with Ddel variants D101, D102, and D404. Variant M301 was a component of two different alleles in the USA, with Ddel variants D201 and D301. Variants M303 and M304, very similar to M301 but found only in South Africa, were associated with Ddel variants D405 and D503 (Figures 1 and 2). In neotropical samples, because of the small difference in fragment sizes that distinguished M301, M303, and M304, the African or European origin of the alleles containing the Mspl-1.8kb fragment was confirmed by the Ddel variant with which they were associated. As in the USA, M301 was found with D201 and D301 in drones in Mexico and Honduras, indicating that the samples were European. In addition, variants D202, D203, and D305, found only in drones from Honduras, had fragments common to variants found in USA