27

Distribution of alleles at locus 178 in Old and New World drone honey bees.

 For each population, the sample size, the total number of alleles

detected, and the number of unique alleles detected are given in Table 1. The

identification and distribution of the 65 different pairwise combinations of

variants, corresponding to alleles, is summarized in Table 2.

 In USA drones, 14 alleles were found that were composed of Mspl

variants from the M100, M200, and M300 groups, and Ddel variants from the

D100, D200, D300, and D400 groups. Mspl variants M101, M201, and

M203, and Ddel variant D401, and a total of eight alleles were unique to USA

drones (Table 2, distribution 1).

 In the South African drones, a total of 19 Mspl variants from the M300,

M400, and M500 groups were detected; 19 Ddel variants from the D300,

D400, and D500 groups were detected. Ten Mspl and ten Ddel variants were

unique to the South African samples. A total of 28 different alleles were

identified, 26 of which were not found in any other population (Table 2,

distribution 8).

 In samples from a single colony in Brazil, near the site of the introduction

and release of the African bees, the Ddel variants identified were D405 and

D504, which also had been found in South African drones (not shown). The

Mspl variants in this colony, and thus the alleles, were not determined.

 In drones from Honduras, a total of 18 Mspl variants, 17 Ddel variants,

and 25 alleles were identified. Alleles in these samples were composed of