22 Similar to the D200 variants, D301-D306 (Figures 4, 5 & 6) contained a 1.25kb fragment, lacked the 1.Okb fragment detected in D101 and D102 but contained a 0.75kb fragment that the D100 and D200 variants lacked (Figures 4, 5 & 6). D301-D304 were individually distinguished by the combinations of fragments between 2.1kb and 2.0kb and between 1.4kb and 1.25kb. D305 was further distinguished by a unique fragment of approximately 1.8kb. D306 contained a 3.4kb fragment and lacked a fragment near 1.1kb found in the other D300 variants. Variants D301-D305 were found in North America and Honduras, while D306 was found only in South Africa. The largest group, D400, consisted of fifteen variants characterized by 1.Okb and 0.75kb fragments (Figures 4 & 7). The D400 alleles differed from each other in the size and intensity of fragments of approximately 1.4kb to 1.3kb and in the absence, or slight variation in the size, of a fragment near 1.1kb. The majority of the D400 alleles were restricted in distribution to the South African and Honduran populations, but two D400 variants were also found in Mexico. The D500 variants were characterized by 0.95kb and 0.75kb fragments (Figures 4 & 5). Each variant in this group differed in its combination of two fragments between 2.1 kb and 2.0kb, two fragments between 1.4kb and 1.3kb, and a single fragment of variable size near 1.1kb. These variants were found in Mexico, Honduras, and South Africa.