6 it is not possible to know the genotype of each queen bee introduced, nor many details of the events contributing to the establishment of New World honey bee populations, some reconstruction can be acheived by studying contemporary bees in the Old and New World. Evidence is provided that reflects the racial composition of Central and South American bees prior to the introduction of African bees, and indicates the occurrence of some, albeit limited, hybridization of European and African bees. Materials and Methods Sources of honey bees. Protocols described by Hall (1986, 1990) for the collection, caste determination, and preservation of bees were followed without modification. Drones were collected as larvae and pupae. South African samples, from four locations in the Transvaal, were collected in January 1990 by HGH. Brood samples from a colony in So Frango, Brasilia, were obtained in 1990 by J. Maruniak, University of Florida. Honduran samples included drones from feral colonies and from managed colonies established from feral swarms, collected in November 1989 by HGH and A. Suazo, Escuela Agricola Panamericana, Honduras. Samples from Tapachula, Mexico, obtained in January 1988 by HGH, were from feral swarms captured in bait hives maintained by the Mexican agency Secretariat of Agriculture and Hydrologic Resources (SARH), and from two managed apiaries. Sources of USA drones included a closed breeding population maintained in Arizona,